{"id":399,"date":"2016-06-15T18:34:23","date_gmt":"2016-06-16T00:34:23","guid":{"rendered":"http:\/\/ciresblogs.colorado.edu\/reccs\/?p=399"},"modified":"2016-06-15T18:55:25","modified_gmt":"2016-06-16T00:55:25","slug":"preparing-lb-agar-plates-and-concentrated-bacillus-solution","status":"publish","type":"post","link":"https:\/\/ciresblogs.colorado.edu\/reccs\/2016\/06\/15\/preparing-lb-agar-plates-and-concentrated-bacillus-solution\/","title":{"rendered":"Preparing LB Agar Plates and Concentrated Bacillus Solution"},"content":{"rendered":"

June 7th<\/sup>, 2016<\/strong><\/p>\n

\"Cleaning<\/a>Today I worked on creating 1\/100 LB agar plates for the experiment. It consisted of weighing out solid compounds with a digital scale, washing agar powder using DI water and the magnetic stirrers, autoclaving the LB agar solution to sterilize it, letting it cool on the magnetic stirring plates, adding antifungals using a PR-1000 pipette, pouring the solution into plates under the flow hood, labelling the plates, and leaving them to solidify overnight. It surprised me how long this process took. It legitimately took me all \"Addition<\/a>day to prepare these plates. It was a fun process, regardless! I never thought I\u2019d be able to prepare agar plates myself. They\u2019ve always been prepared for me in previous lab courses and I never knew how they were made. I\u2019m wondering if any of the procedures I\u2019ve performed so far I\u2019ll have the chance to repeat again. I think it would be good practice and would really help me memorize the technique.
\n\"CLCC<\/a>Tess and I also formed a concentrated bacteria solution that we will later use for extracting peptidoglycan fragments. The bacteria started off in an Erlenmeyer flask labelled, \u201cCLCC 646\u201d which consisted of some kind of solvent and bacillus bacteria. In order to concentrate the bacteria into a small 50 mL plastic tube, we had to pipette them evenly into 6 plastic tubes for centrifuging using an automatic pipette tool. After centrifuging the CLCC 646, pellets formed at the bottom of the tubes making it easy to dump the supernatant off into the sink. We then resuspended the pellets in 1 mL of DI water using the PR-1000. I thought to myself how frustrated I\u2019m going to feel going back to college lab courses after the summer. The tools I\u2019m getting to use throughout the summer are far more convenient, accurate, and efficient than anything I\u2019ve ever used in other labs. I\u2019m certainly going to miss the ease of use!<\/p>\n

I\u2019m looking forward to seeing how we will extract the peptidoglycan fragments from a whole bunch of concentrated cells. Tomorrow I will be touring NOAA, so I\u2019ll likely have to wait until Thursday.<\/p>\n","protected":false},"excerpt":{"rendered":"

June 7th, 2016 Today I worked on creating 1\/100 LB agar plates for the experiment. It consisted of weighing out solid compounds with a digital scale, washing agar powder using DI water and the magnetic stirrers, autoclaving the LB agar solution to sterilize it, letting it cool on the magnetic stirring plates, adding antifungals using… Read More<\/a><\/p>\n","protected":false},"author":79,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[4],"tags":[],"class_list":["post-399","post","type-post","status-publish","format-standard","hentry","category-reccs-2016"],"jetpack_featured_media_url":"","publishpress_future_action":{"enabled":false,"date":"2026-07-30 01:50:49","action":"change-status","newStatus":"draft","terms":[],"taxonomy":"category"},"publishpress_future_workflow_manual_trigger":{"enabledWorkflows":[]},"_links":{"self":[{"href":"https:\/\/ciresblogs.colorado.edu\/reccs\/wp-json\/wp\/v2\/posts\/399","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/ciresblogs.colorado.edu\/reccs\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ciresblogs.colorado.edu\/reccs\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ciresblogs.colorado.edu\/reccs\/wp-json\/wp\/v2\/users\/79"}],"replies":[{"embeddable":true,"href":"https:\/\/ciresblogs.colorado.edu\/reccs\/wp-json\/wp\/v2\/comments?post=399"}],"version-history":[{"count":3,"href":"https:\/\/ciresblogs.colorado.edu\/reccs\/wp-json\/wp\/v2\/posts\/399\/revisions"}],"predecessor-version":[{"id":430,"href":"https:\/\/ciresblogs.colorado.edu\/reccs\/wp-json\/wp\/v2\/posts\/399\/revisions\/430"}],"wp:attachment":[{"href":"https:\/\/ciresblogs.colorado.edu\/reccs\/wp-json\/wp\/v2\/media?parent=399"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ciresblogs.colorado.edu\/reccs\/wp-json\/wp\/v2\/categories?post=399"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ciresblogs.colorado.edu\/reccs\/wp-json\/wp\/v2\/tags?post=399"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}